| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 341 | Bacillus subtilis | TGGCACAGCTGCTTGGAA | CGTGAATCGCATGCGCAA | 59.80 | 59.90 | 186 | 53 |
100.00%
|
100.00%
|
| Primer 342 | Bacillus subtilis | TGGCACAGCTGCTTGGAA | AATCGCATGCGCAACACC | 59.80 | 59.82 | 182 | 53 |
100.00%
|
50.00%
|
| Primer 343 | Bacillus subtilis | AGCTGACAGAGGAGCTTGC | AGCTGCCGTCAAGCTGAT | 59.70 | 59.33 | 291 | 53 |
100.00%
|
100.00%
|
| Primer 344 | Bacillus subtilis | GGACAGGCATTGCGTCAAT | ACAGGCCTTGGTTTGTGACA | 58.82 | 60.03 | 172 | 53 |
100.00%
|
100.00%
|
| Primer 345 | Bacillus subtilis | TGCAGCCGACCGAATCAT | AGAGCATTGCTGTTACGCCT | 59.42 | 60.04 | 170 | 53 |
100.00%
|
100.00%
|
| Primer 346 | Bacillus subtilis | GGCCTGTGGATGACGGAAA | TCTCCGACGAGCTCACTCT | 60.00 | 59.70 | 280 | 52 |
100.00%
|
100.00%
|
| Primer 347 | Bacillus subtilis | ACCGACTTCAGCGCTTGA | AACCGCTGTGTCCGTTCT | 59.27 | 59.18 | 219 | 52 |
100.00%
|
100.00%
|
| Primer 348 | Bacillus subtilis | TGCAGCGAGAACGACTGA | TGTTCCAACCGCACGTCT | 58.96 | 59.50 | 286 | 52 |
100.00%
|
100.00%
|
| Primer 349 | Bacillus subtilis | GCGTTCTGTCCCCAGTTGT | ATGCTGTGTGCCAATCAGGA | 60.23 | 59.96 | 243 | 52 |
100.00%
|
50.00%
|
| Primer 350 | Bacillus subtilis | AGCACAAGGGATGGTCGTG | CACAACCCCTTCAGCCGTA | 60.00 | 59.63 | 265 | 52 |
100.00%
|
100.00%
|